Two-step, PCR-free telomerase detection by using exonuclease III-aided target recycling.
نویسندگان
چکیده
We report the sensitive detection of telomerase activity by using exonuclease III-aided target recycling to amplify the signal produced by a chimeric LNA-DNA molecular beacon. We demonstrate the specific detection of as few as 30 telomerase-positive breast cancer cells in a single-measurement fluorescence assay that avoids the problematic PCR and gel analysis of the current "gold-standard" assay.
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عنوان ژورنال:
- Chembiochem : a European journal of chemical biology
دوره 12 18 شماره
صفحات -
تاریخ انتشار 2011